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KMID : 0896220010210010185
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Journal of Daegu Health College
2001 Volume.21 No. 1 p.185 ~ p.200
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Lymphocytes Mitogenic and Immunochemical Properties of the Lectins(LCC-Ⅲ) from Marine Animal Lunella coronata coreensis
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So Myung-Suk
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Abstract
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A new lectin was purified from whole body of marine shellfish, Lunella coronata coreensis(LCC) by salt fractionation, anionic and cationic ion exchange chromatography. The results were as follows : LCC lectin was relatively stable at the range of pH4-10 and the temperature of 0-50℃. It was dissociated to a single subunit of 22KD by SDS-polyacrylamide gel electrophoresis. In carbohydrates inhibition assay, lectin activity was strongly inhibited by lactose and lactulose at a concentration 6.25mM. LCC lectins(LCC DE 52 0.1M DE 0.2M) were revealed each other as having partial identity by immunochemical studies. LCC lectin proved to be a potent mitogen for murine splenic lymphocytes. And the optimum mitogenic dose was 7.81㎍/㎖.
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